abstract
Investigation into the effects of an antifungal agent on the growth
of equine ringworm in-vitro. (E. Wiggens, F. Ruedisueli)
The sensitivity
of ringworm, (T. mentagrophytes and T. equinum) to the antifungal
agent 'Tolnaftate' incorporated in acrylic fibre was tested in vitro.
In addition, the effect of repeated washing of the fibre on its
effectiveness was tested.
Both T. mentagrophytes
and T. equinum ringworm were cultured on Sabourauds Dextrose
Agar supplemented with the antibacterial agent Chloramphenicol (SDA+C),
for two weeks. The inhibition zone around the fibres was monitored
regularly, and recorded via measurements and photo imaging.
In addition,
T. equinum was cultured on Sabourauds Dextrose Agar which
had the antibacterial agent Chloramphenicol and the antifungal agent
Cycloheximide (SDA+CC) added. The plates were then left to grow
for two weeks. The inhibition zone around the fibre was measured
and recorded.
It was conclusively
found that the acrylic fibre containing the antifungal agent 'Tolnaftate'
inhibited the growth of T. mentagrophytes and T. equinum
cultures in vitro. Furthermore, there was no significant difference
in the size of inhibition zones between T. mentagrophytes
and T. equinum.
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