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Abstract
The inhibitory effects of anti-fungal fibre on Malassezia pachydermatis
in-vitro. (F. Ruedisueli)
Objective
The research objective was investigate the effectiveness of Tolnaftate
containg anti-fungal fibre against the canine yeast pathogen, Malassezia
pachydermatis in-vitro.
Methods
The research entailed the in-vitro culture of M.pachydermatis,
on a Sabouraud Dextrose Agar (SDA) containing chloramphenicol and
cyclohexamide. The control culture was compared to the test cultures
containing 0.5g of anti-fungal fibre. Growth was monitored on a
daily basis and a successful growth of M.pachydermatis was
achieved after 48 hrs of incubation at 30oC. The results were photographically
logged. The Swiss Test grading (SNV 195-920) was used to determine
effectiveness, with grade 1 indicating a good effect with pronounced
inhibition zone and grade 6 indicating full growth with no inhibition
(appendix).
Results
The results are shown in the attached images. Figure 1 and
2 show the control culture with a continuous homogenous growth of
M.pachydermatis across the agar. Figure 3 and 4 show the
test sample, demonstrating an apparent but limited inhibition of
growth compared to the control culture. A small inhibition zone
was seen around the anti-microbial fibre. No fungal growth was observed
under the fibre and in direct contact with the fibre, though the
inhibition zone in some areas appeared almost absent. Therefore,
the results were determined as Grade 2.
Discussion
The results of this study demonstrate that in this instance the
inclusion of 0.5g of anti-fungal fibre in-vitro, did demonstrate
a sufficient inhibiting effect on the growth of M.pachydermatis
in-vitro. The tests were repeated with the same results, indicating
the potential of using anti-fungal fibres to inhibit environmental
contamination with M.pachydermatis.
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